Strategies for Developing NADH Detector Based on Meldola Blue in Different Immobilization Methods: A Comparative Study
نویسندگان
چکیده
Foi realizado um estudo comparativo entre duas metodologias de imobilização do Azul de Meldola (MB), utilizado para diminuir o potencial de oxidação do NADH. O estudo foi realizado no eletrodo de carbono vítreo através da eletropolimerização do pirrol e MB, empregando a técnica de voltametria cíclica e avaliando-se o efeito do número de ciclos para definir a espessura e a estabilidade do filme. Com 25 ciclos foi obtido o melhor resultado. Foi também investigada a modificação do eletrodo de grafite com filme formado pelo sistema sol-gel PPO 300 ou PPO 4000 pelo método de recobrimento por mergulho, através de dois métodos: oclusão e adsorção. O melhor método foi o de adsorção da MB durante 10 min, seguida de depósito do filme PPO 300, favorecido pelo tempo de secagem (24 horas). A adsorção da MB seguido do depósito do PPO 300 apresentou maior intervalo linear do que a metodologia do PPY.
منابع مشابه
Development of a New Ethanol Biosensor with Electropolymerised Meldola Blue as Mediator
An amperometric biosensor for ethanol was developed based on immobilization of alcohol dehydrogenase on screen-printed electrodes modified with electropolymerised Meldola Blue carried out by cyclic voltammetry in the range from –0.6 to +1.4 V vs. Ag/AgCl. The calibration plots for ethanol in 0.1 mol.L-1 phosphate buffer pH 8.5 containing 0.1 M KCl shows linearity in the range from 0.1x10-3 to 2...
متن کاملVoltage Sag in Distribution Systems- A review and comparative study (Reasons, Characteristics, Compensations and Detection)
By quick technological advancements in industrial control processes, major industries demand higher power quality. According to IEEE 1159-1995 standard, any problems in voltage, current or frequency that lead to the error or malfunctions in electrical equipment are considered as a power quality problem. Among the power quality phenomena, voltage sag has been an important problem for power sys...
متن کاملSelective Electrochemical Nanosensor based on Modified Carbon Paste Electrode for Determination of NADH in the presence of Uric Acid
The electrochemical properties of a modified carbon paste electrode with the synthesized compound of 2,2'-[1,7–heptanediylbis(nitrilomethylidene)]-bis(4-hydroxyphenol) (DHBH) and graphite nanoparticle (GN) were studied by cyclic voltammetry (CV), chronoamperometry and differential pulse voltammetry (DPV) methods. The proposed electrode shows excellent electrocatalytic activity towards the oxida...
متن کاملAn Appropriate Theoretical Model for Developing Ambient Air Quality Standard in Iran Based on Standard Setting Approaches of Different Parts of the World
Background & objectives: Environmental standards are quantitative criteria for protecting the environment from pollution and degradation. One of the solutions to improve air quality status is to use reasonable limits to prevent excessive release of airborne contaminants from their various sources. On the other hand, these standards are effective in evaluating air pollution reduction strategies....
متن کاملA Comparative Study of Field Gamma-ray Spectrometry by NaI(Tl) and HPGe Detectors in the South Caspian Region
Natural radionuclides present in soil as well as certain anthropogenic radionuclides released to the environment are the major contributors to terrestrial outdoor exposures. In the assessment of human exposures from environmental radioactivity, besides the conventional method of soil and vegetation sampling combined with laboratory based analyses of environmental media, the other choice would b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006